---
vault_clearance: KETER
halo:
  classification: RESTRICTED
  confidence: HIGH
  front: "12_Project_BloodyEchoes"
  custodian: "Resolve"
  created: 2026-03-24
  updated: 2026-03-27
  wing: CONDITIONAL
  containment: "Unpublished bounties — UHRF1 coordinates, ancient DNA comparison, lifespan reconstruction"
---
# BloodyEchoes — Bounty Board

> **Paradigm: Real History.** The genome is an archaeological site. Interventions live in [18_LifeSong](../18_Project_LifeSong/BOUNTY_BOARD.md). Read the board first; claim before work. Tiers: CLAIM | CONJECTURE | DERIVATION | THEOREM. **Last updated:** 2026-03-27.

---

## SOLVED

| ID | Bounty | One-line answer | Date | Where | Tier |
|----|--------|-----------------|------|-------|------|
| *(none yet)* | | | | | |

---

## OPEN — Cross-Species (the lifespan question)

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| BE-C1 | **Naked Mole Rat UHRF1 Locus** — Compare repeat density in UHRF1-DNMT1 region. NMR (HetGla_female_1.0) lives 30+ years, negligible senescence. Cleaner locus = evidence. | Ready to run | — | **CRITICAL (forum)** |
| BE-C2 | **Bowhead Whale UHRF1 Locus** — Same for bowhead (BalMys1). 200+ years. Longest-lived mammal. | Needs assembly check | — | HIGH |
| BE-C3 | **239-Primate Alignment at UHRF1** — Kuderna et al. 2023 (Nature). Find exact insertion events correlating with lifespan across 239 species. | GCP job | — | HIGH |
| BE-C6 | **Cross-Species det(K) Comparison** — Compute operator coupling determinant for species with different lifespans. Does baseline det(K) predict maximum lifespan? | Dataset hunting | — | HIGH |

---

## OPEN — Ancient DNA (the real history)

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| BE-H1 | **Denisovan UHRF1-DNMT1 TE landscape** — Compare Denisova 3 genome TE insertions in chr19:4.7-10.2 Mb to modern human. Fewer insertions = projected longer lifespan. The genome is the historical record. | Research + computation | — | **CRITICAL** |
| BE-H2 | **Neanderthal UHRF1 comparison** — Vindija 33.19 and Altai Neanderthal. Same analysis. Different species, different TE landscape, different lifespan ceiling? | Research + computation | — | **CRITICAL** |
| BE-H3 | **Date the critical UHRF1 insertion events** — When did AluYf1 and AluYh3 enter UHRF1 introns? Molecular clock dating from the 239-primate alignment. This dates the lifespan drop. | Computation (BE-C3 data) | — | **CRITICAL** |
| BE-H4 | **Pre-Younger Dryas archaic DNA** — Are there archaic human genomes old enough to test? Check ancient DNA databases for samples > 12,000 years. Compare UHRF1 region to modern. | Database survey | — | HIGH |
| BE-H5 | **Ancient text correlation** — Map the dated TE insertion events (BE-H3) against ancient records of lifespan changes. Does the genomic clock match the textual record? | Cross-disciplinary | — | SPECULATIVE but paradigm-defining |

---

## OPEN — Molecular Archaeology

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| BE-C4 | **UHRF1 Splicing Analysis** — Check if intronic AluYf1 creates aberrant splice site. Compare human vs chimp isoforms. | Needs BAM files | — | HIGH |
| BE-C5 | **TE Methylation Age Clock Correlation** — Correlate HERV-Age/LINE-1-Age with UHRF1 protein levels. | Literature + data mining | — | MEDIUM |
| BE-C7 | **L1HS Mapping in UHRF1 Region** — Map ALL L1HS within 1 Mb of UHRF1 and DNMT1. Full-length vs truncated. Orientation. | Ready to run | — | HIGH |

---

## OPEN — Wet Lab (validation)

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| BE-W1 | **UHRF1 Overexpression in Senescent ECs** — If cascade attenuated, UHRF1 is the bottleneck. | Needs plasmid | — | HIGH |
| BE-W2 | **Alu Excision from UHRF1 Intron** — CRISPR flanking AluYf1 at chr19:4,919,307. Does UHRF1 expression increase? | Hard | — | HIGH |
| BE-W4 | **Fasting Mimetic on ECs** — Does UHRF1 increase with rapamycin/serum starvation? | Rapamycin available | — | MEDIUM |

---

## Priority Order

### Before forum (April 13)
1. **BE-C1** (NMR comparison) — one GCP job, definitive for the talk
2. **BE-C7** (L1HS mapping) — can run today
3. **BE-H1** (Denisovan comparison) — the headline historical claim

### After forum
4. BE-H3 (date the insertions) — when did the lifespan drop?
5. BE-C3 (239-primate) — comprehensive
6. BE-H2 (Neanderthal) — second species comparison
7. BE-W1 (UHRF1 overexpression) — wet lab validation

---

## RETRACTED / WONTFIX

| ID | What | Why |
|----|------|-----|
| BE-T1 through BE-T5, BE-W3, BE-W5, BE-W6 | Temporal protocol bounties | Moved to [18_Project_LifeSong](../18_Project_LifeSong/BOUNTY_BOARD.md) — interventions live there, history lives here |