---
vault_clearance: KETER
halo:
  classification: RESTRICTED
  confidence: MEDIUM
  front: "18_Project_LifeSong"
  custodian: "Resolve"
  created: 2026-03-27
  updated: 2026-03-27
  wing: NOT_READY
  containment: "Temporal biology bounties — intervention timing, reprogramming protocols"
---
# LifeSong — Bounty Board

> **Current Tier: Vault** | **Orthodox Cultivator:** Standard Yamanaka (bulk OSKM, constitutive expression) | **Target: Consensus**
> **FORM question:** Does WHEN matter more than WHAT in biological reprogramming? **Our answer:** Temporal structures from LENG lotus time predict intervention timing.

> **Paradigm: Temporal Biology.** The intervention is the rhythm. Read the board first; claim before work. Tiers: CLAIM | CONJECTURE | DERIVATION | THEOREM. **Last updated:** 2026-03-27.

---

## SOLVED

| ID | Bounty | One-line answer | Date | Where | Tier |
|----|--------|-----------------|------|-------|------|
| *(none yet)* | | | | | |

---

## OPEN — Temporal Mathematics

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| LS-M1 | **Map expression oscillation periods (T) for cascade genes** — UHRF1, DNMT1, TRIM28, SETDB1 each have a characteristic T. Measure from time-course scRNA-seq. t_L = 9T/2 predicts the intervention window per gene. | Needs time-course data | — | **CRITICAL** |
| LS-M2 | **Validate lotus time against known biology** — Does t_L predict: methylation maintenance cycle, UHRF1 half-life, cell cycle checkpoint durations, neural binding window, anesthetic onset? Systematic comparison across scales. | Computation | — | **CRITICAL** |
| LS-M3 | **Biological force hierarchy** — Map LENG petal traversal depths (0/7/14) to biological intervention depths (gene expression / photon-mediated / systemic hormesis). Does (η/p)⁷ suppression ratio appear in biological dose-response curves? | Theory + data | — | HIGH |
| LS-M4 | **Temporal asymmetry in biology** — K - η = 4/9 is the cost of reversal in LENG. What is the biological analog? Is rejuvenation always harder than aging by a fixed ratio? Measure from senescence/rejuvenation time-course data. | Computation | — | HIGH |

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## OPEN — Protocol Design

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| LS-P1 | **Design reverse-cascade Yamanaka protocol** — Specify: which factor, dose, duration (t_L), order (TRIM28→DNMT1→UHRF1→decouple). Literature scan for pulsed delivery systems (doxycycline-inducible, optogenetic, mRNA pulses). | Research | — | **CRITICAL** |
| LS-P2 | **Literature scan: pulsed vs constitutive Yamanaka** — Find all papers comparing pulsed vs continuous partial reprogramming. What pulse widths? Does intermittent beat continuous? Anyone report timing-dependent outcomes? | Research | — | **CRITICAL (before forum)** |
| LS-P3 | **PBM + cascade timing** — Does pulsed PBM (10/40 Hz) at t_L-matched frequency enhance epigenetic reversal more than continuous? Theoretical prediction from LENG force hierarchy. | Theory | — | HIGH |
| LS-P4 | **Hormesis periodization protocol** — Map fasting/cold/exercise cycles to circadian t_L. Optimal window: how long to fast, when to break, how cold, for how long? Command/Orem framework applied. | Cross-project (07) | — | MEDIUM |

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## OPEN — Hardware

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| LS-H1 | **Build PBM panel v1** — 660nm + 850nm LEDs, Arduino timer, 10/40 Hz PWM, ~50 mW/cm². Bill of materials, build instructions, calibration protocol. Under $50. | BUILD | — | HIGH |
| LS-H2 | **Calibrate PBM panel** — Measure actual irradiance at treatment surface. Solar power meter or Thorlabs sensor. Map dose (J/cm²) vs distance vs time. | Needs panel + meter | — | HIGH |

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## OPEN — Wet Lab

| ID | Bounty | Status | Assigned | Impact |
|----|--------|--------|----------|--------|
| LS-W1 | **PBM on senescent ECs** — 660nm + 850nm, pulsed 10 Hz. Measure: ATP, UHRF1, det(K), SA-β-gal, p21. Pre/post. | Needs PBM panel | — | HIGH |
| LS-W2 | **Pulsed Yamanaka on ECs** — Dox-inducible OSKM, reverse cascade order. Compare: constitutive vs pulsed (t_L-timed) vs random. Measure: rejuvenation markers, methylation age, UHRF1. | Hard | — | **CRITICAL** |
| LS-W3 | **PBM + hormesis synergy** — PBM before/during/after cold exposure or fasting mimetic (rapamycin). Does combination > either alone? Measure UHRF1, det(K), senescence markers. | Needs panel + reagents | — | MEDIUM |

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## Priority Order

### Before forum (April 13)
1. **LS-P2** (literature scan: pulsed Yamanaka) — know what's been tried
2. **LS-M2** (validate lotus time) — one computation, foundational claim

### After forum
3. LS-M1 (oscillation periods) — needs data
4. LS-P1 (reverse-cascade design) — the actual protocol
5. LS-H1 (build PBM panel) — tangible deliverable
6. LS-W1 (PBM on ECs) — first wet lab test
7. LS-W2 (pulsed Yamanaka) — the big experiment

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## RETRACTED / WONTFIX

| ID | What | Why |
|----|------|-----|
| *(none)* | | |